Question
How monoclonal antibodies are typically produced using hybridoma technology?
Answer :
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Monoclonal antibodies (mAbs) are produced using hybridoma technology, which involves the fusion of a specific antibody-producing B lymphocyte with a myeloma cell to create a hybrid cell line capable of indefinite growth and antibody production. The process begins with the immunization of a suitable animal, usually a mouse, with the antigen of interest. This stimulates the mouse’s B cells to produce antibodies against the antigen. After several booster injections, B cells are harvested from the spleen, as these cells contain the genetic information to produce the desired antibody. The harvested B cells are
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