Question
ELISA
Answer :
Word Count : 254
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Enzyme-Linked Immunosorbent Assay (ELISA) is a sensitive and widely used immunological technique employed to detect and quantify antigens or antibodies in biological samples such as serum, plasma, saliva, or cell culture supernatants. The method is based on the specific interaction between an antigen and its corresponding antibody, combined with an enzyme-mediated color change that allows visual or spectrophotometric measurement. ELISA plays a crucial role in diagnostics, research, and epidemiological studies due to its accuracy, simplicity, and adaptability. The basic principle involves immobilizing either an antigen or antibody onto a solid surface, usually a polystyrene microtiter plate. A specific antibody or antigen linked
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