Question
Describe steps in cloning eukaryotic insulin genes in prokaryotes for recombinant human insulin production using synthetic A/B chains and fusion proteins.
Answer :
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The production of recombinant human insulin in prokaryotes involves cloning the insulin gene, either by synthesizing separate A and B chains or using fusion proteins to facilitate expression. Initially, the nucleotide sequences coding for the A and B chains of insulin are determined and chemically synthesized according to the human insulin sequence. These synthetic genes are designed with appropriate codon optimization to ensure efficient expression in prokaryotic hosts like *Escherichia coli*. Each chain is individually
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