Question
Elucidate the different techniques of western blotting.
Answer :
Word Count : 246
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Western blotting is an important analytical technique used in genomics and proteomics to detect and characterize specific proteins within a complex biological sample. The process begins with protein extraction, where proteins are isolated from cells or tissues using appropriate lysis buffers that preserve protein integrity. The extracted proteins are then separated based on their molecular weight using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), in which SDS denatures proteins and imparts a
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