Question
Explain the technique of nuclear run-on assay with diagram.
Answer :
Word Count : 270
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The nuclear run-on assay is a molecular biology technique used to measure the transcriptional activity of specific genes by detecting newly synthesized RNA. This assay helps distinguish between transcriptional regulation and post-transcriptional regulation of gene expression, making it a valuable tool in genomics and proteomics studies. The procedure begins with the isolation of nuclei from cells under conditions that preserve ongoing transcription. Once isolated, the nuclei are incubated with labeled nucleotides, typically radioactive or fluorescently tagged, allowing RNA polymerases that are already engaged on DNA to extend their transcripts. This “run-on” step incorporates the labeled nucleotides into nascent RNA, marking transcripts that were actively being
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