Question

Explain with a suitable diagram the mechanism of action of lysozyme. 

14 May 2026
Answer :
Word Count : 466
Lysozyme is a hydrolytic enzyme widely distributed in egg white, tears, saliva, mucus, and many animal tissues. It acts as an antibacterial agent by destroying the cell wall of bacteria, especially Gram-positive bacteria. The enzyme catalyzes the hydrolysis of β-(1→4) glycosidic linkage between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) present in peptidoglycan of bacterial cell walls. As a result, the integrity of the bacterial wall is lost, leading to osmotic lysis and death of the cell. Lysozyme is a small globular protein containing a deep cleft or groove that serves as the active site. The substrate binds in this groove through several weak interactions. The active site contains two important amino acid residues: Glutamic acid-35 (Glu-35) and Aspartic acid-52 (Asp-52). These residues play a major role in catalysis. The bacterial
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