Question
Explain physical adsorption method of enzyme immobilizationv
Answer :
Word Count : 299
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Physical adsorption is the simplest and oldest method of enzyme immobilization. In this method, enzymes are attached to the surface of insoluble carriers or matrices by weak physical forces such as van der Waals forces, hydrogen bonds, hydrophobic interactions, ionic interactions, and electrostatic forces. No covalent bond is formed between the enzyme and the support material. The enzyme gets adsorbed on the carrier surface under suitable conditions of pH, temperature, and ionic strength. Various materials are used as carriers for adsorption. These include activated charcoal, alumina, silica gel, glass beads, cellulose, starch, collagen, agarose, and ion-exchange resins. The support material should have a large surface area, good mechanical strength, chemical stability, and biocompatibility for
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