Question
Explain the role of DNA ligase in joining DNA fragments and differentiate between sticky and blunt ends.
Answer :
Word Count : 250
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Skip in 10
DNA ligase plays a crucial role in joining DNA fragments during molecular cloning, DNA replication, and repair processes. It catalyzes the formation of phosphodiester bonds between the 3' hydroxyl group of one DNA strand and the 5' phosphate group of another. This enzymatic action effectively seals the "nicks" in the sugar-phosphate backbone of
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