IGNOU BBCCT 125 2026 - Describe the principle of Sanger's DNA sequencing method

Question

Describe the principle of Sanger's DNA sequencing method.

09 Feb 2026

Answer :

Word Count : 227

Sanger's DNA sequencing method, also known as the dideoxy chain termination method, relies on the selective incorporation of chain-terminating nucleotides to determine the nucleotide sequence of DNA. The principle of this technique involves using a single-stranded DNA template, a primer, DNA polymerase, normal deoxynucleotide triphosphates (dNTPs), and modified dideoxynucleotide triphosphates (ddNTPs). The ddNTPs, which lack a 3'-OH group, prevent

_____ ______ ___ ________ ______ _____ ________.
___ _____ __________ _____ _______ _________ _______ ___ __________ ________ __________ ___.
_________ _______ _______ ________ _________ ______.
_______ ___ ____ ___ ________ _____ _______ _________ ____.
________ ________ ______ ___ ______ _______.
________ ___ ___ ________ _____ ______ ____ ______ _________.
___ __________ ____ _____ ___ __________ _____ _______ _______ _________.
_________ _____ _________ __________ ___ ______ _________ __________ ____ __________ ____.
____ __________ __________ ________ _____ __________ ___ ____.
__________ ______ __________ ____ ____ _____ _____ ________ ____ ___ ________.
___ _____ ____ __________ _______ _____ _____ ________ _______ ________ ___ __________.
__________ _____ __________ ___ _____.
____ __________ ___ _________ _________ _______ _______ ______ ____ _______ _________ _______.
____ ____ ______ ______ _______ _________ ________ _____ ____.
_______ _____ ______ ____ _______ ____ _____ _____ _____.
_______ _________ ____ ________ ____ ____ _______.
________ ____ ______ ____ _____.
_____ _____ ___ ________ ______.
______ _______ ____ _____ ____ _________ _________ ___ ________ ___ _____ _____.
___ ________ __________.

IGNOU ACADEMY

Your IGNOU journey, made easier.

Solved Assignments
Study Notes
Guess Papers
Question Papers

Call Us WhatsApp

STUDY • PREPARE • SUCCEED

Skip in 10

IGNOU Solved Assignments Assignments Ignou Projects Project Previous Year Solved Exam Papers Notes Notes Sample Question Papers Sample Question Papers