IGNOU BBCCT 125 2026 - Explain the principles of insertional inactivation and blue-

Question

Explain the principles of insertional inactivation and blue-white selection.

09 Feb 2026

Answer :

Word Count : 197

Insertional inactivation is a molecular technique used in genetic engineering to identify recombinant DNA molecules. It relies on disrupting a functional gene or marker through the insertion of foreign DNA. A common target for this process is the *lacZ* gene, which encodes the enzyme β-galactosidase,

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