Question
Explain the use of electroporation for DNA transfer into animal cells and its advantages over chemical methods.
Answer :
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Electroporation is a widely used method for transferring DNA into animal cells by applying an electrical field to temporarily permeabilize the cell membrane, allowing the entry of DNA molecules. When cells are exposed to a brief electric pulse, small pores form in the cell membrane, enabling the passage of genetic material into the cytoplasm. This method is particularly useful for difficult-to-transfect cell types and is a popular choice for introducing plasmids, siRNA, or other nucleic acids into mammalian cells. One of the key advantages of electroporation over chemical methods, such as lipofection
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