Question
Explain the challenges in producing recombinant proteins in E. coli and the role of fusion tags in purification.
Answer :
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Producing recombinant proteins in *Escherichia coli* is widely used due to its fast growth, well-characterized genetics, and cost-effectiveness, but it faces several challenges. One major issue is the formation of inclusion bodies, where expressed proteins aggregate in insoluble forms due to misfolding, often caused by the high expression rate or
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