Question
Explain how real-time PCR is used for quantitative analysis of gene expression.
Answer :
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Real-time PCR, also known as quantitative PCR (qPCR), is a powerful technique used to measure gene expression levels in real-time. It is based on the amplification of specific DNA sequences, which allows for the quantification of target genes during the amplification process. The technique uses fluorescent dyes or probes that bind to the DNA and emit fluorescence when the DNA is amplified. The fluorescence intensity correlates with the amount of DNA present in each cycle of PCR, enabling the measurement of gene expression. In real-time
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