IGNOU BBCCT 125 2026 - Describe how linkers, adapters, and synthetic oligonucleotid

Question

Describe how linkers, adapters, and synthetic oligonucleotides are used to clone blunt-ended DNA fragments.

09 Feb 2026

Answer :

Word Count : 274

Cloning blunt-ended DNA fragments often requires specialized techniques because blunt ends lack the sticky overhangs that facilitate easy ligation. Linkers, adapters, and synthetic oligonucleotides are molecular tools used to modify blunt-ended DNA fragments to enable their cloning. Linkers are short, double-stranded DNA sequences with a known restriction enzyme recognition site. When blunt-ended fragments are treated with linkers, these linkers are ligated onto the ends of the DNA fragments. The added restriction sites allow subsequent digestion with the appropriate restriction

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