Question
ई. कोलाई में पुनर्संयोजित प्रोटीन के उत्पादन में आने वाली चुनौतियों और शुद्धिकरण में संलयन टैग की भूमिका की व्याख्या कीजिए।
Answer :
Word Count : 229
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Skip in 10
ई. कोलाई में पुनर्संयोजित प्रोटीन का उत्पादन कई चुनौतियों के साथ जुड़ा होता है। सबसे बड़ी चुनौती यह है कि प्रोटीन अक्सर गलत फोल्डिंग या इन्सोल्यूबल फॉर्म में जमा हो जाता है, जिसे inclusion bodies कहा जाता है। इसके अलावा, ई. कोलाई में प्रोटीन पर post-translational modifications जैसे glycosylation नहीं
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