Enzymes are biological catalysts that accelerate biochemical reactions without being consumed during the reaction. Due to their specificity and sensitivity, enzymes play a significant role in medical diagnostics and analytical devices such as enzyme electrodes. Enzyme diagnostics involves the measurement of enzyme activity in body fluids and tissues to detect diseases, assess organ function, and monitor treatment. Enzyme electrodes, on the other hand, are biosensing devices that combine enzymes with electrochemical transducers for the rapid estimation of various metabolites. In clinical diagnostics, enzymes are valuable indicators of tissue damage and pathological conditions. Many enzymes are normally present inside cells, and their levels in blood increase when tissues are damaged. Measurement of serum enzyme levels helps in identifying the affected organ and the severity of disease. For example, elevated levels of alanine aminotransferase and aspartate aminotransferase indicate liver disorders such as hepatitis and cirrhosis. Similarly, alkaline phosphatase levels rise in bone diseases and obstructive jaundice. Creatine kinase and lactate dehydrogenase are important diagnostic markers for myocardial infarction and