Question
Describe SDS-PAGE technique.
Answer :
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SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis) is a widely used bioanalytical technique for separating proteins based on their molecular weight. The technique combines the denaturing properties of SDS with the sieving effect of a polyacrylamide gel. SDS, an anionic detergent, binds uniformly to protein molecules, giving them a negative charge proportional to their length and effectively masking their native charge. This ensures that during electrophoresis, the migration of proteins is determined primarily by size rather than intrinsic charge or shape. Before loading onto
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