Question
Gas Chromatography and HPLC
Answer :
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Gas Chromatography (GC) and High-Performance Liquid Chromatography (HPLC) are essential bioanalytical techniques widely used for the separation, identification, and quantification of compounds in complex biological samples. Gas Chromatography is primarily applied for volatile and thermally stable compounds. In GC, the sample is vaporized and carried by an inert gas, such as helium or nitrogen, through a column coated with a stationary phase. Different components interact with the stationary phase to varying degrees, causing separation based
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