Question
Describe MABS and Cloning Vectors.
Answer :
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Monoclonal antibodies (MABS) are highly specific antibodies produced by a single clone of B-lymphocyte cells. They recognize and bind to a single antigenic determinant or epitope. MABS are produced by hybridoma technology developed by Köhler and Milstein. In this method, an animal such as a mouse is immunized with a specific antigen. Antibody-producing spleen cells are isolated and fused with immortal myeloma cells using polyethylene glycol. The fused cells, called hybridomas, possess the ability to produce antibodies continuously. Hybridomas are selected using HAT medium and screened for desired antibody
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