Question
Explain the principle of ammonium sulphate fractionation and dialysis in protein purification.
Answer :
Word Count : 206
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Ammonium sulfate fractionation is a technique used to precipitate proteins based on their solubility. When ammonium sulfate is gradually added to a protein solution, the solubility of the proteins decreases as the ionic strength increases. Proteins with varying solubility at different ammonium sulfate concentrations can be separated. At a specific concentration, proteins aggregate and precipitate out of the solution. This allows for the selective precipitation of target proteins while leaving impurities in the supernatant. The precipitated proteins are then collected by centrifugation,
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