IGNOU BBCCT 105 2026 - Describe the principles of ion-exchange, gel filtration, and

Question

Describe the principles of ion-exchange, gel filtration, and affinity chromatography.

09 Feb 2026

Answer :

Word Count : 259

Ion-exchange chromatography separates proteins based on their net surface charge at a particular pH. Proteins carry positive or negative charges depending on their amino acid composition and the surrounding pH. In cation-exchange chromatography, negatively charged resins bind positively charged proteins, whereas anion-exchange chromatography uses positively charged resins to bind negatively charged proteins. Proteins are then eluted by gradually changing the ionic strength or pH of the buffer, which disrupts the electrostatic interactions between the protein and the resin. The principle relies on the differential affinity of proteins for the charged matrix,

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