IGNOU BBCCT 105 2026 - Design a purification strategy using chromatographic t

Question

Design a purification strategy using chromatographic techniques to isolate an enzyme from a crude protein extract.

09 Feb 2026

Answer :

Word Count : 265

To isolate an enzyme from a crude protein extract using chromatographic techniques, a systematic purification strategy is essential. The process usually begins with selecting appropriate methods based on the enzyme’s properties such as size, charge, hydrophobicity, and affinity for specific ligands. First, start with ammonium sulfate precipitation to concentrate and partially purify the enzyme by exploiting its solubility differences. After precipitation and dialysis to remove salts, proceed with ion exchange chromatography. This method separates proteins based on their net charge

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