Question
Describe the basic theory, components and applications of thin layer chromatography.
Answer :
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Thin layer chromatography (TLC) is a widely used bioanalytical technique based on the principle of separation of components in a mixture according to their relative affinities toward stationary and mobile phases. The basic theory relies on adsorption and partitioning. In TLC, the stationary phase is a thin layer of adsorbent material, usually silica gel or
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