IGNOU BBCCT 105 2026 - Explain how SDS-PAGE, isoelectric focusing, and 2-D electrop

Question

Explain how SDS-PAGE, isoelectric focusing, and 2-D electrophoresis can be used to analyze complex protein mixtures.

09 Feb 2026

Answer :

Word Count : 243

SDS-PAGE, isoelectric focusing, and 2-D electrophoresis are widely used techniques for analyzing complex protein mixtures, each offering unique advantages in protein separation. SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a technique that separates proteins based on their molecular weight. In this process, proteins are denatured by SDS, which imparts a negative charge proportional to their size.

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