IGNOU BBCCT 105 2026 - Describe methods used for determining protein purity and mol

Question

Describe methods used for determining protein purity and molecular weight.

09 Feb 2026

Answer :

Word Count : 301

Several methods are employed to determine protein purity and molecular weight, each with its own set of advantages depending on the sample type and required sensitivity. One of the most common methods for assessing protein purity is SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis). This technique separates proteins based on their size after they have been denatured by SDS, which gives them a negative charge. The protein samples are loaded onto a gel, and an electric field is applied, causing proteins to migrate toward the positive electrode. The distance a protein travels depends on its molecular

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