IGNOU MCH 2 2018 - What is gradient elution? How is it useful? Discuss.

Question

What is gradient elution? How is it useful? Discuss.

08 Mar 2018

Answer :

Word Count : 309

Steady changes of the mobile phase composition during the chromatographic run is called gradient elution. It may be considered as an analogy to the temperature programming in gas chromatography.

The main purpose of gradient elution is to move strongly retained components of the mixture faster, but having the least retained component well resolved.

Starting with the low content of the organic component in the eluent

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