Explain the preparative group preparations of size exclusion chromatography
High-resolution size-exclusion chromatography (SEC) is not often used at large scales compared with other chromatography modes because of the restrictions in sample size and flow rate. In this article, the authors provide application data to support the use of SEC beyond small-scale operations, using the tentacle resin Fractogel EMD BioSEC (S). The separation of 20 and 40 kDa proteins was illustrated in a case study, in which flow rate and sample size were varied to examine the operational limits with regard to productivity. In a second application, efficient aggregate removal in monoclonal antibody purification was demonstrated. With SEC as the second step after Protein A affinity capture, the monomer with a purity of 99.5% was obtained. Purity
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