IGNOU AEC 1 2018 - Describe the principle of ion exchange chromatography. Give one of its

Question

Describe the principle of ion exchange chromatography. Give one of its applications

06 Mar 2018

Answer :

Word Count : 325

Ion exchange chromatography is a process that allows the separation of ions and polar molecules based on their affinity to the ion exchanger. It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids.

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Principle:

Ion Exchange chromatography principle, Exchange of ions is the basic principle in this type of Chromatography. In this process two types of exchangers i.e., cationic and anionic exchangers can be used.

Cationic exchangers possess negatively charged group, and these will attract positively charged cations. These exchangers are also called “Acidic ion exchange” materials, because their negative

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