Urea of concentration 0.1mM was hydrolysed using increase of concentration of 10.0 micro mole. Using the values k2 = 6 × 104s-1 and Michaelis constant for urease 2.0 m M. Calculate the initial rate of the reaction
To calculate the initial rate of the hydrolysis reaction of urea, we can use the Michaelis-Menten equation, which relates the initial rate of an enzyme-catalyzed reaction to the substrate concentration and enzyme kinetic parameters. The Michaelis-Menten equation is given by:
\[V_0 = \frac{{V_{\text{max}}[S]}}{{K_m + [S]}}\]
Where:
- \(V_0\) is the initial rate of the reaction.
- \(V_{\text{max}}\) is the maximum reaction rate when the enzyme is saturated with substrate.
- \([S]\) is the substrate concentration.
- \(K_m\) is the Michaelis constant, which represents the substrate concentration at which the reaction rate is half of \(V_{\text{max}}\).
In this
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