Proteolytic enzymes, also known as proteases or proteinases, are enzymes that catalyze the hydrolysis of peptide bonds in proteins, thereby breaking complex protein molecules into smaller peptides and amino acids. They play a crucial role in numerous biological processes such as digestion, metabolism, cell regulation, immune responses, blood coagulation, and tissue remodeling. Proteolytic enzymes are widely distributed in plants, animals, microorganisms, and even viruses. Based on their site of action and catalytic mechanism, they are broadly classified into endopeptidases, which cleave peptide bonds within the protein chain, and exopeptidases, which remove amino acids from the terminal ends of proteins. One of the most important proteolytic enzymes is pepsin, which is found in the gastric juice of the stomach. Pepsin is secreted in an inactive form called pepsinogen and becomes active in the acidic environment of the stomach. It primarily acts on dietary proteins, breaking them down into smaller polypeptides and peptides. Pepsin shows maximum activity at low pH and is essential for the initial stages of protein digestion in humans and other animals. Trypsin is another well-known proteolytic