Question
PCR based mutagenesis
Answer :
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PCR-based mutagenesis is a molecular biology technique used to introduce specific mutations into a DNA sequence using the polymerase chain reaction (PCR). This method allows precise changes, such as substitutions, deletions, or insertions, in a target gene without the need for naturally occurring mutations. The process involves designing synthetic oligonucleotide primers that carry the desired mutation. During PCR, these primers anneal to the template DNA and guide the DNA polymerase to incorporate the mutation into the newly synthesized strands. There
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