Question
How cDNA library differs from genomic library? Explain.
Answer :
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A genomic library and a complementary DNA (cDNA) library differ fundamentally in their source material, composition, and applications in recombinant DNA technology. A genomic library is constructed using the total genomic DNA of an organism. The DNA is isolated from cells, fragmented using restriction enzymes, and cloned into suitable vectors. As a result, a genomic library represents the entire genome of an organism, including coding regions (exons), non-coding regions (introns), regulatory sequences, repetitive DNA, and intergenic regions. In contrast, a cDNA library is constructed from messenger RNA (mRNA) molecules extracted from a specific cell
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