Question
Explain the steps and application of FISH technique with suitable diagram.
Answer :
Word Count : 269
___ ___ ________ ______ ___ _________ _____ __________.
____ ___ _______ _____ _______.
______ _______ ________ ______ __________ _________ __________ ____ __________.
__________ _____ _________ ___ ______ _______ ___ ____ ______ ______.
____ ___ ________ ____ _______ _______ _______ _______ ___.
_______ ___ ____ ____ ___ ___ ___ ___ ___ ___ __________.
_________ ______ _______ __________ _____ ____ _____ _____ __________ _______ _______ ____.
_______ _________ ____ __________ ________ ________ ___ ______.
______ _____ ________ _____ ______ ___ _______ ___ _______ _______ ________ ____.
______ ___ ______ _______ _______ __________.
____ __________ __________ ___ ________.
____ __________ _________ _______ __________ ____ ___ __________ ________ _________ ____.
_________ _________ _______ ______ __________ ______ ______ ___ ____.
____ __________ _______ ______ _________.
_______ ___ _____ __________ ____ ______.
____ _________ _________ ___ ____ _________ _____ _________ __________ _________.
________ __________ __________ _________ ___ ___ ____ ___ ________ __________ ____.
______ _________ ________ _______ ______ ______ ____ ______.
_____ ______ ____ ____ ________ _______.
Skip in 10
Fluorescence in situ hybridization (FISH) is a powerful cytogenetic technique used to detect and localize specific DNA sequences on chromosomes. The first step in the FISH procedure is sample preparation, which involves obtaining metaphase chromosome spreads from dividing cells or using interphase nuclei. The chromosomes are then fixed onto a glass slide and treated with protease or detergent to permeabilize the cells, making the DNA accessible for probe binding. Next, denaturation is performed where both the chromosomal DNA and the fluorescently labeled DNA probe are heated to separate the double strands into single strands. This is a critical step because
____ ____ _________ __________ ______ _____ ____ __________.___ ___ ________ ______ ___ _________ _____ __________.
____ ___ _______ _____ _______.
______ _______ ________ ______ __________ _________ __________ ____ __________.
__________ _____ _________ ___ ______ _______ ___ ____ ______ ______.
____ ___ ________ ____ _______ _______ _______ _______ ___.
_______ ___ ____ ____ ___ ___ ___ ___ ___ ___ __________.
_________ ______ _______ __________ _____ ____ _____ _____ __________ _______ _______ ____.
_______ _________ ____ __________ ________ ________ ___ ______.
______ _____ ________ _____ ______ ___ _______ ___ _______ _______ ________ ____.
______ ___ ______ _______ _______ __________.
____ __________ __________ ___ ________.
____ __________ _________ _______ __________ ____ ___ __________ ________ _________ ____.
_________ _________ _______ ______ __________ ______ ______ ___ ____.
____ __________ _______ ______ _________.
_______ ___ _____ __________ ____ ______.
____ _________ _________ ___ ____ _________ _____ _________ __________ _________.
________ __________ __________ _________ ___ ___ ____ ___ ________ __________ ____.
______ _________ ________ _______ ______ ______ ____ ______.
_____ ______ ____ ____ ________ _______.
IGNOU ACADEMY
Your IGNOU journey, made easier.
- Solved Assignments
- Study Notes
- Guess Papers
- Question Papers
Continue Reading — It's Free
Sign in with Google to unlock more of this answer.
✓ Free access
✓ Unlock up to 60% of the answer
✓ No payment required
✓ Unlock up to 60% of the answer
✓ No payment required
Quick Google sign-in. No separate registration form.