Explain the principle of revolving can shutter system used for simultaneous measurement of fluorescence and phosphorescence
Once suitable sampling procedure has been achieved the sample in principle can be used for the simultaneous measurement of fluorescence and phosphorescence as the two emissions occur at different wavelengths. However, the fluorescence emission may cause interference in the measurement of phosph orescence. This interference can be eliminated by taking advantage of the fact that once the incident radiation is cut off the fluorescence ceases immediately whereas the pho
sphorescence emission continues. A mechanical shutter can be used to execute recording of phosphorescence under these conditions. One commonly used mechanical shutter system consists of a revolving can that surrounds the sample and has a small window. The can is so rotated that when it is opposite the excitation monochromator it allows a short burst of radiation to fall on the sample and immediately cut off. After a certain time delay it allows the radiation emitted during phosphorescence
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