The fluorescence emission occurs at longer wavelengths than the absorption. Explain with the help of an energy level diagram
Absorption of light occurs very quickly (approximately a femtosecond, the time necessary forthe photon to travel a single wavelength) in discrete amounts termed quanta and corresponds to excitation of the fluorophore from the ground state to an excited state. Likewise, emission of a photon through fluorescence or phos
phorescence is also measured in terms of quanta. The energy in a quantum ( Planck's Law ) is expressed by the equation:
E = hn = hc/l
where E is the energy, h is Planck's constant, n and l are the frequency and wavelength of the incoming photon, and c is the speed of light. Planck's Law dictates that the radiation
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