Question
Explain the different mechanisms in fluorescence quenching.
Answer :
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Fluorescence quenching is the process by which the fluorescence intensity of a molecule decreases due to various physical or chemical interactions. It plays an important role in atomic and molecular spectroscopy for studying molecular structure, interactions, and environmental conditions. One of the most common mechanisms is dynamic or collisional quenching. In this process, the excited fluorophore collides with a quencher molecule during its excited-state lifetime. The collision transfers the excitation energy into non-radiative forms, reducing fluorescence intensity. The efficiency of dynamic quenching depends on temperature, viscosity, and the
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