IGNOU MCH 4 2017 - Explain methodology of radioimmunoassay and list its advantages.

Question

Explain methodology of radioimmunoassay and list its advantages.

17 Jun 2017

Answer :

Word Count : 726

Radioimmunoassay (RIA): Methodology and Advantages

Radioimmunoassay (RIA) is a sensitive and specific laboratory technique used to quantitatively measure the concentration of specific antigens (usually hormones, drugs, or other molecules) in biological samples. It relies on the principles of immunology and radioactive isotopes, making it a powerful tool in biochemistry, clinical medicine, and research. In this method, a radiolabeled antigen competes with a non-labeled antigen for binding to a limited number of antigen-specific antibodies. The amount of radiolabeled antigen that competes successfully with the unlabeled antigen indicates the concentration of the antigen of interest in the sample.

Methodology of Radioimmunoassay:

1. Antibody Preparation: The first step involves generating highly specific antibodies that can recognize and bind to the target antigen. These antibodies are typically produced in animals (e.g., rabbits or mice) by immunization with the antigen of interest. The animals' immune systems produce antibodies against the

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