IGNOU BBYET 143 2026 - Describe the procedure for construction of Genomic and cDNA libraries.

Question

Describe the procedure for construction of Genomic and cDNA libraries.

31 Jan 2026

Answer :

Word Count : 488

Genomic and cDNA libraries are essential tools in molecular biology, used to store large quantities of genetic material from an organism. The construction of these libraries involves different procedures based on the type of genetic material being analyzed—genomic DNA or complementary DNA (cDNA). To construct a genomic library, the first step is to extract high-quality genomic DNA from the organism of interest. This DNA is then fragmented into smaller pieces using physical (e.g., sonication) or enzymatic methods (e.g., restriction enzymes). These fragments are then ligated into vectors such as plasmids or bacteriophages, which are capable of replicating within a host cell, typically *E. coli*. The ligated vectors are then introduced into bacterial cells through a process called transformation or transduction, depending on

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