Ozone layer depletion is a significant environmental issue that has attracted global attention due to its widespread impacts on the biosphere. The ozone layer, situated in the stratosphere at an altitude of approximately 15 to 35 kilometers above the Earth’s surface, serves as a protective shield by absorbing the majority of the sun’s harmful ultraviolet (UV) radiation, particularly UV-B and UV-C rays. This layer is composed of ozone (O₃) molecules, which are formed naturally through the interaction of solar ultraviolet radiation with molecular oxygen (O₂). The presence of the ozone layer is crucial for maintaining ecological balance and protecting living organisms from the deleterious effects of excessive UV exposure. The depletion of the ozone layer occurs primarily due to human-induced activities that release ozone-depleting substances (ODS) into the atmosphere. Key contributors include chlorofluorocarbons (CFCs), halons, carbon tetrachloride, and methyl chloroform, which are commonly used in refrigeration, air conditioning, foam production, and fire extinguishing agents. Once released, these substances rise to the stratosphere, where they undergo photodissociation under UV light, releasing chlorine and bromine atoms. These free radicals act as catalysts in the destruction of ozone molecules through chain reactions, where one chlorine atom can destroy thousands of ozone molecules before being deactivated. This leads to a thinning of the ozone layer, with the most notable manifestation being the seasonal “ozone hole” observed over Antarctica. The consequences of ozone layer depletion are profound and multifaceted, affecting both the abiotic and biotic components of the biosphere. One of the most direct impacts is the increase in ultraviolet-B radiation reaching the Earth’s surface. Elevated UV-B levels have severe biological effects on living organisms. For humans, this manifests as an increased risk of skin cancers, including melanoma and non-melanoma types, cataracts, and other eye disorders. Prolonged exposure also suppresses the immune system, reducing the ability to fight infections and increasing vulnerability to diseases. The effects are particularly pronounced in populations with high sun exposure and in regions where the ozone layer thinning is more significant. For terrestrial ecosystems, higher UV-B radiation can adversely affect plant growth, photosynthesis, and crop