IGNOU LSE 1 2015 - Describe the methods for separating macro molecules on the basis of mo

Question

Describe the methods for separating macro molecules on the basis of molecular size, solubility and molecular affinity.

23 Dec 2017

Answer :

Word Count : 425

A number of sophisticated analytical and preparative techniques have been developed for separating, analyzing, and isolating the various macromolecular constituents of cells and tissues.

Various forms of electrophoresis, chromatography, and ultracentrifugation are now in routine use in most laboratories engaged in molecular biological studies and have greatly increased our understanding of the chemistry and properties of the cellular macromolecules.

Most of the methods that are used to separate and isolate different members of a class of macromolecules simultaneously provide information concerning their chemistry because parameters such as molecular size, shape, density, net and absolute charge, differential solubility, and so forth are used as the basis for the separation.

Nearly all the techniques that are used for separating and isolating

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