Question
Describe how overlapping peptides are generated and used to determine the complete amino acid sequence of a protein.
Answer :
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Overlapping peptides are generated through a process called fragmentation, where a protein is cleaved into smaller peptides that overlap with adjacent ones. This method allows the determination of the complete amino acid sequence of a protein by ensuring that sequence information from one peptide can be used to deduce the sequence of the next one. To generate overlapping peptides, a protein is typically cleaved using specific enzymes, such as trypsin, which cuts the protein at specific amino acid sequences.
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