Question
What are the characteristics of stationary phases used in liquid-solid chromatography? Discuss various types of adsorbents used.
Answer :
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In liquid–solid chromatography, the stationary phase is a solid adsorbent that remains fixed in the column while the liquid mobile phase passes through it. The stationary phase plays a crucial role in separation by selectively adsorbing components of the mixture based on differences in polarity, molecular size, and intermolecular interactions. An ideal stationary phase should have a large surface area to provide maximum adsorption sites, uniform particle size for consistent flow and efficient separation, mechanical strength to withstand pressure, chemical inertness
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