Question
Explain the principle of DNA sequencing techniques.
Answer :
Word Count : 258
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DNA sequencing refers to the determination of the exact order of nucleotides present in a DNA molecule. The fundamental principle behind all DNA sequencing techniques is the generation of DNA fragments whose terminal nucleotide can be identified, followed by their separation and detection to deduce the original sequence. Most sequencing methods rely on complementary base pairing, DNA polymerase activity, and controlled termination or detection of nucleotide incorporation. In classical Sanger sequencing, also
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