Question
Explain the internal standard method as quantitative analysis methodology used in flame photometry
Answer :
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The measurement Of calcium in biological materials by flame photometry is more difficult than that of sodium and potassium because of its lower emission energy and more serious interference by contaminat- ing substances. The first difficulty can overcome by using a high flame temperature, a prism mono- chromator, and a sensitive detector. Instruments incorporating these features permit the use of the weak calcium line at 422-7 mg where interference is less than using the calcium oxide bands at 554 mg and 622 mp. Even at 422-7 rnp, however, there are
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