Describe the isotope dilution analysis.
Isotope dilution analysis is a method of determining the quantity of chemical substances. In its most simple conception, the method of isotope dilution comprises the addition of known amounts of isotopically-enriched substance to the analyzed sample. Mixing of the isotopic standard with the sample effectively "dilutes" the isotopic enrichment of the standard and this forms the basis for the isotope dilution method. Isotope dilution is classified as a method of internal standardisation, because the standard (isotopically-enriched form of analyte) is added directly to the sample. In addition, unlike traditional analytical methods which rely on signal intensity, isotope dilution employs signal ratios. Owing to both of these advantages, the method of isotope dilution is regarded among chemistry measurement methods of the highest metrological standing.
Isotope dilution can be effectively explained using mark and recapture method from biology - a method commonly used in ecology to estimate the population size of fish.
Isotope dilution can be likened to Lincoln-Petersen method. Assume that the number of fish in a pond is to be determined. Five
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