What is gradient elution? How is it useful? Discuss.
Steady changes of the mobile phase composition during the chromatographic run is called gradient elution. It may be considered as an analogy to the temperature programming in gas chromatography.
The main purpose of gradient elution is to move strongly retained components of the mixture faster, but having the least retained component well resolved.
Starting with the low content of the organic component in the eluent we allow the least retained components to be separated. Strongly retained components will sit on the adsorbent surface on the top of the column, or will move very slowly.
When we start to increase an amount of organic component in the eluent (acetonitrile) then strongly retained components will move
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