Question

Describe principle and procedure used for the Atomic Absorption Spectroscopy (AAS) in food analysis.

06 Feb 2018
Answer :
Word Count : 811

The selectivity in AAS is very important, since each element has a different set of energy levels and gives rise to very narrow absorption lines. Hence, the selection of the monochromator is vital to obtain a linear calibration curve (Beers' Law), the bandwidth of the absorbing species must be broader than that of the light source; which is difficult to achieve with ordinary monochromators. The monochromator is a very important part of an AA spectrometer because it is used to separate the thousands of lines generated by all of the elements in a sample.

Without a good monochromator, detection limits are severely compromised. A monochromator is used to select the specific wavelength of light that is absorbed by the sample and to exclude other wavelengths. The selection of the specific wavelength of light allows for the determination of the specific element of interest when it is in the presence of other elements. The light selected by the monochromator is directed onto a detector,

typically a photomultiplier tube, whose function is to convert the light signal into an electrical signal proportional to the light intensity. The challenge of requiring the bandwidth of the absorbing species to be broader than that of the light source is solved with radiation

sources with very narrow lines.

The study of trace metals in wet

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