The fluorescence emission occurs at longer wavelengths than the absorption. Explain with the help of an energy level diagram
Absorption of light occurs very quickly (approximately a femtosecond, the time necessary forthe photon to travel a single wavelength) in discrete amounts termed quanta and corresponds to excitation of the fluorophore from the ground state to an excited state. Likewise, emission of a photon through fluorescence or phos
phorescence is also measured in terms of quanta. The energy in a quantum ( Planck's Law ) is expressed by the equation:
E = hn = hc/l
where E is the energy, h is Planck's constant, n and l are the frequency and wavelength of the incoming photon, and c is the speed of light. Planck's Law dictates that the radiation energy of an absorbed photon is directly proportional to the frequency and inversely proportional to the wavelength, meaning that shorter incident wavelengths possess a greater quantum of energy. The absorption of a photon of energy by
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