IGNOU BCHET 141 2026 - How does surface area of the stationary phase affect the separation in

Question

How does surface area of the stationary phase affect the separation in coloumn chromatography? What should be the sample to absorbent ratio so that clear separations are obtained?

10 Jan 2026

Answer :

Word Count : 265

In column chromatography, the surface area of the stationary phase plays a critical role in the separation of components. A stationary phase with a higher surface area provides more active sites for adsorption of the sample molecules, which increases the interaction between the solute and the stationary phase. Stronger interactions result in slower movement of the analytes through the column, allowing better resolution between components with similar properties. Conversely, a stationary phase with low surface area reduces adsorption, causing the solutes to move too quickly and potentially leading to overlapping peaks and poor separation. Therefore, choosing an appropriate stationary phase with sufficient surface

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