Question
How does determination of nucleic acid base composition help in microbial characterization?
Answer :
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Determination of nucleic acid base composition, particularly the proportion of guanine plus cytosine (G+C content) in DNA, plays an important role in microbial characterization and classification. Different microorganisms possess distinct and relatively stable G+C percentages, which reflect their genetic makeup. By estimating base composition, microbiologists can compare organisms at the molecular level and identify similarities or differences that may not be evident from morphology or physiology alone. The G+C content is usually expressed as a molar percentage and is determined using techniques such as thermal denaturation, buoyant density analysis, or modern molecular methods. Closely related microbial
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